Check Interaction. The aims of this study were to determine the optimal incubation time needed for the absorption of toluidine blue by cells of C. albicans, C. glabrata, C. kruseiand C. The overall sensitivity and specificity of toluidine blue were 51.4 and 84.6 respectively. We used the cell membrane-impermeant ferricyanide ion as a secondary electron acceptor to measure the extracellular reduction of methylene blue independently from its (D and I) Quantification of toluidine blue uptake by the aerial parts of young The sensitization In the dysplastic and malignant lesions the false negatives were 2%, and there were 30% false positives in the

The whole point of toluidine blue is that it is a stain, which means it is applied and then excess removed (we used Surgilube as I mentioned). by MUSTAFA ZTRK. a) Do NOT apply to mucus membranes because the uptake will be diffuse and nonspecific for injury. Photocytotoxicity and dark toxicity of toluidine blue-O. 1974 Apr;189(1):221-34.

The corresponding values for identifying high-risk PMD (lesions with moderate and severe dysplasia) using acetic acid were 100%, 9.1%, 25.9%, and 100%, respectively, and for Chemically Toluidine blue is a basic thiazine metachromatic dye having high affinity for acidic tissue components like DNA and RNA thus serves in detecting tissues rich in former mentioned To assess the histological features of in vivo toluidine blue (TB) uptake in potentially malignant oral lesions (PML) and to determine whether any were related to clinical Mary Carroll, B.A.

Toluidine blue uptake by dead parasites was very fast. Assays were then placed back in the incubator. All krs Right: Toluidine blue-highlighted laceration after application of the dye and destaining by spraying with 1% acetic acid. It was J Pharmacol Exp Ther.



The purpose of the present study was to evaluate two thiazine compounds, methylene blue (MB) and toluidine blue O (TBO), as electron acceptor probes for the Several dyes are used extensively in ophthalmic surgery. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. Overall accuracy of the toluidine blue uptake was 91 %. A method for measuring the uptake of toluidine blue by bacteria on membrane filters was developed. Toluidine blue (TB; active ingredient: o-tolonium chloride, 3-amino-7-dimethylamino-2-methylphenazothionium chloride) is a redox-active, cationic, metachromatic

uptake efficiency by developing cuticles is an essential strategy for plant survival. This was based mainly on the effective protection Bacteria were filtered out of solution onto a cellulose acetate filter and The toluidine blue was displaced by protamine sulfate because protamine sulfate has higher affinity for carboxyl groups and sulfate groups in the glycocalyx than toluidine blue.

SDS Toluidine Blue Stain O Page 1 of 7! 1998;68(3):370-376. Interactions between cationic dyes and negatively charged mineral surfaces have long attracted great attention from clay mineralogists, environmental scientists, and A mucosal tongue stained blue is observed. Overall accuracy of the toluidine blue uptake was 91%. Interaction of toluidine blue and rat mast cells: histamine release and uptake and release of the dye. 21, 23 when a 1% aqueous tb solution is Toluidine blue (also known as tolonium chloride) is an acidophilic metachromatic dye that selectively stains acidic tissue components (sulfates, 2.

Cuticle limits gas exchange but does not completely shut it off.

Toluidine blue (vital staining) also is a useful adjunct to clinical examination and biopsy.

The use of radio-iodinated toluidine blue for myocardial scintigrams Edward A. Carr, Jr., M.D.

Abstract A method for measuring the uptake of toluidine blue by bacteria on membrane filters was developed. 1. (Jones, et al., 2004) Signs of genital trauma in adolescent rape victims In the dysplastic and malignant lesions, the false negatives were 2 %, and there were 30 % false positives in the

It is also used to stain proteoglycans and glycosaminoglycans in tissues such as

We hypothesised that PBAEs capabilities to actively deliver molecules inside cells could be exploited as adjuvant to microbial cell uptake of toluidine blue O (TBO) and 1974 Apr;189(1):221-34. It's not so much meant to J Pharmacol Exp Ther.

it has been reported that toluidine blue stains premalignant and malignant lesions, but, not the benign lesions and normal mucosa.

Chui C,

Express a drop of the aspirate in the center of the slide.

Use of toluidine blue in tissue sections is done with the aim to highlight components, such as mast Interactions between cationic dyes and negatively charged mineral surfaces have long attracted great attention from clay mineralogists, environmental scientists, and chemical

This was based mainly on the effective protection In this report, we compare the interactions of

of toluidine blue in revealing the dysplastic oral mucosal tissues, the wide range of sensitivity and specificity values of toluidine blue Toluidine blue uptake in potentially malignant oral A clear delimitation between the affected mucosa and the rest is

Bhatti M, MacRobert A, Meghji S, Henderson B,

In the dysplastic and malignant lesions the false negatives were 2%, and there were 30% false positives in the benign lesions. As a phenothiazine basic dye, toluidine blue (TB) is commonly used in biotechnology to stain tissues rich in DNA and RNA due to its high affinity for tissue

Photochem Photobiol. A study of the uptake of toluidine blue O by Porphyromonas gingivalis and the mechanism of lethal photosensitization. Overall accuracy of the toluidine blue uptake was 91%. It mostly also partially stained damaged but not dead organisms. The uptake of methylene blue (MB), and toluidine blue O (TBO) by bovine pulmonary arterial endothelial cells grown on microcarrier beads was detected as a decrease and dogs, a significant selecti\-e uptake of ITB by Toludine Blue Dye Used to highlight lacerations that are seen by colposcope Deep blue uptake is interrupted: positive for laceration injury Solution dyes nucleate squamous cells in The stain was positive in 26% (5 of 19) of nondysplastic OPLs, Its main disadvantage was rapid, nonspecific staining of live The clinical diagnosis of the Request PDF | On Apr 1, 2005, M. Pentenero and others published PD.42 In vivo Toluidine blue uptake in oralpotentially malignant lesions: Clinical and histological

Conclusions Both the techniques have adjunctive utility in the diagnosis of dysplasia in

Mechanism of uptake of a cationic water-soluble pyridinium zinc phthalocyanine across the outer membrane of Escherichia coli.

toluidine blue.

In (B) mucosal appearance after adding Lugols solution.

Bacteria were filtered out of solution onto a cellulose acetate filter and

Toluidine blue uptake was associated significantly with degree of dysplasia, consistent with the literature. 1. 2012, European Journal of Obstetrics &

Overall accuracy of the toluidine blue uptake was 91%.

It was

b) Avoid dye entering the vaginal vault although DNA evidenced will be preserved. Toluidine blue uptake quantifications were consistent with these observations, showing significant differences in uptake between wild-type cotyledons and all krs alleles .

Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in DNA and RNA.

Overall accuracy of the toluidine blue uptake was 91%.

The detection of vaginal lacerations in reported rape victims increased from one in 24 to 14 in 24 with toluidine application. 3,4 Toluidine blue (ToB) is an acidophilic metachromatic dye that stains abnormal tissue dark royal blue by penetrating into Less often, the doctor might wipe the vulva with a dye (called toluidine blue) to find areas of abnormal vulvar skin to biopsy.

86 Figure 34. Toluidine blue [TB] also known as tolonium chloride is a characteristic acidophilic metachromatic dye that selectively stains acidic tissue components like sulfates, neoplastic cells will lead in By MUSTAFA ZTRK.

After 24 h, assays were fixed in 3% paraformaldehyde for immunostaining or 3% glutaraldehyde for toluidine blue staining. A previous study reported that the cuticle Overdose. A reason for the use of toluidine blue staining in the presurgical management of patients with oral squamous cell carcinomas.

Scale bars, 200 mm. Put on disposable gloves to handle patient samples. Abstract Toluidine blue, a thiazine dye, was shown to have in vivo photodynamic activity through singlet oxygen (O21g) production. Thus, it seems that

an antidote for methemoglobinemia.

b Representative toluidine blue staining showed the osteoblasts on postero-lateral (PL) periosteal and endosteal surfaces of 37% diaphysis, for both loaded and contralateral,

Overall accuracy of the toluidine blue uptake was 91%. 3. The efficacy of 1% toluidine blue in the identification of oral malignancies and potentially malignant oral lesions was evaluated among a group of Asian patients (n=102) with

Abstract Toluidine blue, a thiazine dye, was shown to have in vivo photodynamic activity through singlet oxygen (O21g) production.

from significance of toluidine blue positive findings after speculum examination for sexual assault (jones, et al., 2004): before the insertion of a speculum or examining fingers, a It has found wide

Toluidine blue is often used to identify mast cells, by virtue of the heparin in their cytoplasmic granules. In the dysplastic and malignant lesions the false negatives were 2%, and there were 30% false positives in the benign lesions. There was no morphological characterization of these erythrocytes and their sialic acid content and the toluidine blue uptake were not lower than in late agglomeration.



In the dysplastic and malignant lesions the false negatives were 2%, and there were 30% false positives in the benign lesions. It was Antibacterial Photodynamic Treatment of Porphyromonas gingivalis with Toluidine Blue O and a NonLaser Red Light Source Enhanced by Dihydroartemisinin Chao Toluidine blue uptake ethnicity (83% versus 81% Caucasian), and smoking history (64% was associated significantly with degree of dysplasia, consistent versus 72%; all comparisons For toluidine blue it was 42.4%, 88.23%, 87.50%, 44.11%, and 58% respectively. To assess the histological features of in vivo toluidine blue (TB) uptake in potentially malignant oral lesions (PML) and to determine whether any were related to clinical In order to investigate the potential effects, postcoital vaginal swabs were taken 6 h after sexual intercourse and exposed directly to 1% toluidine blue in aqueous solution, 1-10% acetic acid,

Rinse gently with 3 Bacteria were filtered out of solution onto a cellulose acetate filter and

In the dysplastic and malignant lesions the false negatives were 2%, and there were 30% false positives in the Antibacterial Photodynamic Treatment of Porphyromonas gingivalis with Toluidine Blue O and a NonLaser Red Light Source Enhanced by Dihydroartemisinin Chao Ding. Stain with 0.04% Toluidine Blue Solution for 10 min 3. Version 2 Revision Date 07/03/2014 Product name: Toluidine Blue Stain Product Number: Brand: Edvotek Supplier: Edvotek 1121 5th Street NW

Procedure for Using Toluidine Blue with Fine-Needle Aspirates 1.

Toluidine Blue / Fast Green Stain PROCEDURE: 1.

The histological findings of areas with toluidine blue uptake and with less-intense staining of Lugols solution were specialized intestinal metaplasia with low-grade dysplasia in two patients Significance of toluidine blue positive findings after speculum examination for sexual assault. Oral Diseases (2012) 18, 728733 Background: The value of chairside adjunctive tests in the detection of oral potentially malignant disorders (OPMDs) remains uncertain. Lauber and Souma, in 1982, It stains nucleic acids blue and polysaccharides purple and also increases the The exact mechanism for the uptake of methylene blue in epithelial tissue may resemble that of toluidine blue in the acidophilic characteristic of cells with abnormal concentration of nucleic 30100 Telegraph Road, Suite 408, Bingham Farms, Michigan 48025 (USA) (A to C, E to H, and J, and K) Toluidine blue tests on etiolated cotyledons. If someone has overdosed and has serious symptoms such as passing out or trouble breathing , call 911.

Deparaffinize slides and rehydrate to deionized water 2. Toluidine blue staining in the diagnosis of endometrial pathologies: a preliminary study before chromohysteroscopy.

A statistically-significant association was observed between the toluidine blue

A method for measuring the uptake of toluidine blue by bacteria on membrane filters was developed. Six levels of toluidine blue staining were differentiated for the West Indian manatee: (1) no stain uptake (Figure 2A Sperm chromatin maturity, or nuclear maturation, was assessed with a (A to C, E to H,andJ,andK) Toluidine blue tests on etiolated cotyledons. (D and I) Quantification of toluidine blue uptake by the aerial parts of young seedlings, Purpose of review To review the literature on toluidine blue (TBlue) and to discuss the utility of TBlue in assessing and in clinical management of patients with oral

Application of toluidine blue dye and its subsequent removal from Toluidine blue has been used in vivo to identify dysplasia and carcinoma of the oral cavity.

It was found that 0.16 mg 125I-toluidine blue gave a concentration ratio of parathyroid/thyroid and parathyroid/muscle which was significantly higher than both lower and higher doses at 1 A study of the uptake of toluidine blue O by Porphyromonas gingivalis and the mechanism of lethal photosensitization.

Toluidine blue staining in the diagnosis of endometrial pathologies: a preliminary study before chromohysteroscopy. There was no morphological characterization of these erythrocytes and their sialic acid content and the toluidine blue uptake were not lower than in late agglomeration.

85 Figure 33.

Gandolfo S, Pentenero M, Broccoletti R, Pagano M, Carrozzo M, Scully C. Toluidine blue uptake in potentially malignant oral lesions in vivo: clinical and histological discussion to the best of our knowledge, this is the first pro- results spective study to analyze the in vivo tissue and cel- lular uptake of toluidine blue (tb) both from a eighteen lesions in 18 This dye causes skin with certain diseases -- including VIN and

illumination. In vivo Toluidine blue uptake in oral potentially malignant lesions: clinical and by radiotherapy, two of them relapsed locally and were rescued with total laryngectomy; one patient received no In order to further investigate this phenotype, we developed a method of quantifying Toluidine Blue uptake by seedlings grown in vitro, and stained for a defined Normann E, Normann T. Scand J Clin Lab Invest,

2006; 102:

Photocytotoxicity and dark toxicity of 9-methyl azure A.

Walter DiGiulio, M.D.

Thus, it Uptake of H-labelled commercial toluidine blue and subfractions (thionine, toluidine blue, and azur A) in the parathyroids of rabbits. Toluidine blue is an important tool to detect and document genital and perianal injuries after sexual assault. Photocytotoxicity and dark toxicity of 1,9-dimethyl

Toluidine blue increases the detection of perineal lacerations in

J Oral Pathol Med (2011) 40: 300304 Introduction: The purpose of this study was to evaluate the efficacy of the toluidine blue (TB) test as a diagnostic tool in the detection of Purpose of review To review the literature on toluidine blue (TBlue) and to discuss the utility of TBlue in assessing and in clinical management of patients with oral Otherwise, call a poison control center right away. 4)

The mechanism is based on selective binding of the dye to dysplastic or malignant cells in the oral

Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components. Figure 3: A study subject from Group B subjected to diagnostic test (toluidine blue + chemiluminescence) and reference standard (histopathology). Scale bars, 200 m. INTRODUCTION.

Toluidine blue uptake in potentially malignant

Interaction of toluidine blue and rat mast cells: histamine release and uptake and release of the dye.